Damage of granulocytes in filtration leukapheresis (FL)

Damage of granulocytes in filtration leukapheresis (FL).

To The Editor: To elucidate the mechanism of granulocyte damage during FL, studies have been reported of the interaction between nylon-wool fibers and isolated granulocytes suspended in protein free media,’2 in 5% plasma,2 or in 1% human serum albumin.3 In FL, however, heparinized whole blood is passed through a nylon-wool filter, resulting in plasma protein coating of the fibers within seconds...

Filtration leukapheresis (FL) and granulocytes.

Detection, pathogenesis, and prevention of damage to human granulocytes caused by interaction with nylon wool fiber. Implications for filtration leukapheresis.

Granulocytes collected by reversible adhesion to nylon wool fiber (NWF) function relatively well in standard in vitro tests; however, they have an abnormally shortened survival time in the circulation. Assuming that this rapid disappearance represents clearance and that recognition by phagocytes is important for such clearance, we used an autologous in vitro cell:cell recognition assay to deter...

Pretreatment of filtration leukapheresis donors with colchicine.

Although filtration leukapheresis (FL) is a relatively simple and efficient means of harvesting neutrophils (PMN) for transfusion, the PMN obtained by this procedure have both functional and morphologic abnormalities induced when the cells adhere to nylon wool filters used in FL. Adherence to nylon wool causes a partial degranulation of PMN together with the production of superoxide at cell sur...

In vitro function and post-transfusion survival of granulocytes collected by continuous-flow centrifugation and by filtration leukapheresis.

The function of granulocytes collected by continuous-flow centrifugation (CFC) and by filtration leukapheresis (FL) was studied in vitro, and the post-transfusion recovery and intravascular survival of these cells was studied by autologous transfusion in normal donors. Granulocytes collected by both FL and CFC leukapheresis (CFCL) functioned normally in the quantitative nitroblue tetrazolium, o...